Anticorps Polyclonal de lapin anti-NDUFB7

NDUFB7 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14912-1-AP

Synonymes

B18, Cell adhesion protein SQM1, CI B18, Complex I B18, NDUFB7

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Galerie de données de validation

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  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14912-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14912-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14912-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14912-1-AP (NDUFB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14912-1-AP

  IP Result of anti-NDUFB7 (IP:14912-1-AP, 3ug; Detection:14912-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14912-1-AP (NDUFB7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 14912-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14912-1-AP (NDUFB7 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, cellules MCF-7, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain, tissu de cancer du col de l'utérus humain, tissu hépatique humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

14912-1-AP cible NDUFB7 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFB7 Protéine recombinante Ag6709
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18-22 kDa
• Numéro d’acquisition GenBank: BC002595
• Symbole du gène: NDUFB7
• Identification du gène (NCBI): 4713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFB7(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7) is also named as CI-B18 and belongs to the complex I NDUFB7 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which couples the oxidation of NADH to the reduction of ubiquinone and the translocation of protons across the inner membrane. NDUFB7 protein lacks a mitochondrial targeting signal and transmembrane domains. However, it has a Cx(9)C motif that includes an intermembrane space targeting signal.(PMID:21310150).

Publications

Species	Application	Title
mouse	WB	Mol Metab; A disease-associated Aifm1 variant induces severe myopathy in knockin mice.; Authors - Lena Wischhof
mouse	WB	Cell Death Dis; Loss of apoptosis-inducing factor critically affects MIA40 function.; Authors - K Meyer
human,mouse	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
	WB	Mol Med; Altered redox mitochondrial biology in the neurodegenerative disorder fragile X-tremor/ataxia syndrome: use of antioxidants in precision medicine.; Authors - Gyu Song
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang