Anticorps Polyclonal de lapin anti-NDUFB8

• Phare
• Validé par KD/KO

NDUFB8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (1)

N° de cat : 14794-1-AP

Synonymes

ASHI, CI ASHI, Complex I ASHI, NDUFB8

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Galerie de données de validation

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  WB analysis using 14794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of various samples using 14794-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

• 

  WB analysis of A549 using 14794-1-AP

  WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.

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  IP experiment of HepG2 using 14794-1-AP

  IP result of anti-NDUFB8(IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug.

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  IHC staining of human liver using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14794-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules 3T3-L1, cellules A549, échantillons divers, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 34 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

14794-1-AP cible NDUFB8 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFB8 Protéine recombinante Ag6495
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC000466
• Symbole du gène: NDUFB8
• Identification du gène (NCBI): 4714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu
mouse	WB	J Exp Med; FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics.; Authors - Yujing Yin
mouse	WB	Biomaterials; Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.; Authors - Xiao-Tao He
pig	WB	J Cachexia Sarcopenia Muscle; MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.; Authors - Ming Qi
mouse	WB	Cell Rep; Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.; Authors - Tingting Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate NDUFB8 works well, both with ECL detection and fluorescent secondary antibodies it gives a strong band

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: K562 and KCL22