Anticorps Polyclonal de lapin anti-NDUFB8

• Phare
• Validé par KD/KO

NDUFB8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14794-1-PBS

Synonymes

ASHI, CI ASHI, CIASHI, CI-ASHI, Complex I-ASHI

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Galerie de données de validation

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  WB analysis using 14794-1-AP (same clone as 14794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  WB analysis of various samples using 14794-1-AP (same clone as 14794-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14794-1-AP (same clone as 14794-1-PBS)

  WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

  IP result of anti-NDUFB8 (IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug. This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14794-1-AP (same clone as 14794-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14794-1-AP (NDUFB8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14794-1-AP (same clone as 14794-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14794-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14794-1-PBS cible NDUFB8 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFB8 Protéine recombinante Ag6495
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC000466
• Symbole du gène: NDUFB8
• Identification du gène (NCBI): 4714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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