Anticorps Monoclonal anti-NDUFB8

• Phare
• Validé par KD/KO

NDUFB8 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

3B12C6

Avis (1)

N° de cat : 67690-1-Ig

Synonymes

ASHI, CI ASHI, Complex I ASHI, NDUFB8

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Galerie de données de validation

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  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67690-1-Ig

  WB result of NDUFB8 antibody (67690-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.

• 

  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

67690-1-Ig cible NDUFB8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFB8 Protéine recombinante Ag6569
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 18-22 kDa
• Numéro d’acquisition GenBank: BC000466
• Symbole du gène: NDUFB8
• Identification du gène (NCBI): 4714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
mouse	WB	Chin J Nat Med; Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway; Authors - Long Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

Good and strong antibody, but with some non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2, mouse liver and mouse brain

NDUFB8 Antibody Western Blot validation (1:8000 dilution) in HepG2, mouse liver and mouse brain (Cat no:67690-1-Ig)