Anticorps Recombinant de lapin anti-NDUFB8

• Phare
• Validé par KD/KO

NDUFB8 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230510B10

N° de cat : 83216-3-PBS

Synonymes

230510B10, ASHI, CI ASHI, CIASHI, CI-ASHI

---

Galerie de données de validation

• 

  WB analysis using 83216-3-RR (same clone as 83216-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 83216-3-RR (same clone as 83216-3-PBS)

  WB result of NDUFB8 antibody (83216-3-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells. This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

  IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug. This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 83216-3-RR (same clone as 83216-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR (same clone as 83216-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83216-3-RR (same clone as 83216-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83216-3-RR (same clone as 83216-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83216-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83216-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 0.177 nM.

• 

  Affinity and Kinetic Characterization of 83216-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

---

Informations sur le produit

83216-3-PBS cible NDUFB8 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NDUFB8 Protéine recombinante Ag6495
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC000466
• Symbole du gène: NDUFB8
• Identification du gène (NCBI): 4714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}