Anticorps Monoclonal anti-NDUFS1

NDUFS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H5E8

N° de cat : 68253-1-PBS

Synonymes

EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD, CI 75Kd

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Galerie de données de validation

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  WB analysis using 68253-1-Ig (same clone as 68253-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68253-1-Ig (same clone as 68253-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68253-1-Ig (NDUFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68253-1-Ig (same clone as 68253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68253-1-Ig (NDUFS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68253-1-Ig (same clone as 68253-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68253-1-Ig (same clone as 68253-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDUFS1 antibody (68253-1-Ig, Clone: 3H5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68253-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68253-1-PBS cible NDUFS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFS1 Protéine recombinante Ag3135
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 727 aa, 79 kDa
• Poids moléculaire observé: 68-81 kDa
• Numéro d’acquisition GenBank: BC030833
• Symbole du gène: NDUFS1
• Identification du gène (NCBI): 4719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

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