Anticorps Polyclonal de lapin anti-NDUFS3

• Phare
• Validé par KD/KO

NDUFS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15066-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 15066-1-AP (same clone as 15066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15066-1-AP (same clone as 15066-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15066-1-AP (NDUFS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 15066-1-AP (same clone as 15066-1-PBS)

  IP result of anti-NDUFS3 (IP:15066-1-AP, 4ug; Detection:15066-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15066-1-AP (same clone as 15066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15066-1-AP (NDUFS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15066-1-AP (same clone as 15066-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NDUFS3 antibody (15066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15066-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

15066-1-PBS cible NDUFS3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS3 Protéine recombinante Ag7072
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000617
• Symbole du gène: NDUFS3
• Identification du gène (NCBI): 4722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

{{ptg:RelatedPrimaryAntibodies}}