Anticorps Recombinant de lapin anti-NDUFS3

NDUFS3 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251654E6

N° de cat : 86676-1-PBS

Synonymes

CI 30kD, CI30kD, CI-30kD, Complex I-30kD, EC:7.1.1.2

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Galerie de données de validation

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  WB analysis using 86676-1-RR (same clone as 86676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86676-1-RR (NDUFS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86676-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86676-1-RR (same clone as 86676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS3 antibody (86676-1-RR, Clone: 251654E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86676-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02593-1

  The mean NDUFS3 concentration was determined to be 89.8 ng/mL in HEK-293T cell extract based on a 1.3 mg/mL extract load, 74.3 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02593-1

  Cytometric bead array standard curve of MP02593-1, NDUFS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86676-2-PBS. Detection antibody: 86676-1-PBS. Standard: Ag7072. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86676-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86676-1-RR against Human NDUFS3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86676-1-PBS cible NDUFS3 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NDUFS3 Protéine recombinante Ag7072
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000617
• Symbole du gène: NDUFS3
• Identification du gène (NCBI): 4722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420).

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