Anticorps Polyclonal de lapin anti-NDUFS4

• Phare
• Validé par KD/KO

NDUFS4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15849-1-AP

Synonymes

AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4

---

Galerie de données de validation

• 

  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15849-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

15849-1-AP cible NDUFS4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS4 Protéine recombinante Ag8633
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC005270
• Symbole du gène: NDUFS4
• Identification du gène (NCBI): 4724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial), also known as AQDQ, is a member of NADH dehydrogenase (ubiquinone) iron-sulfur (NDUFS) protein family. It is a peripheral membrane protein located on the matrix side of the inner mitochondrial membrane. It is reported that the loss of NDUFS4 protein expression in immunohistochemistry (IHC) reliably diagnoses papillary thyroid carcinoma(PMID: 34792302). Also, NDUFS4 promotes tumor progression and predicts prognosis in gastric cancer(PMID: 36044738).

Publications

Species	Application	Title
mouse	WB	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian ; KO Validated
Mouse	WB	Aging Dis; MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.; Authors - Chunling Xue
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
mouse	WB	EBioMedicine; SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.; Authors - Yipeng Du
rat	WB	Arch Biochem Biophys; Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction.; Authors - Qiuhong Mou
		Cell Commun Signal; The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation; Authors - Yunjie Liu

{{ptg:RelatedPrimaryAntibodies}}