Anticorps Polyclonal de lapin anti-NDUFS5

• Phare
• Validé par KD/KO

NDUFS5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15224-1-AP

Synonymes

CI 15 kDa, Complex I 15 kDa, NDUFS5

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Galerie de données de validation

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  WB analysis using 15224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15224-1-AP (NDUFS5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15224-1-AP

  WB result of NDUFS5 antibody (15224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS5 transfected HEK-293 cells.

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  IHC staining of human liver using 15224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15224-1-AP (NDUFS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules L02, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

15224-1-AP cible NDUFS5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS5 Protéine recombinante Ag7441
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC001884
• Symbole du gène: NDUFS5
• Identification du gène (NCBI): 4725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150).

Publications

Species	Application	Title
human		Genes Dev; ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.; Authors - Yueh-Lin Tsai
mouse	WB	Neurosci Bull; Intermittent Theta Burst Stimulation Attenuates Cognitive Deficits and Alzheimer's Disease-Type Pathologies via ISCA1-Mediated Mitochondrial Modulation in APP/PS1 Mice; Authors - Yang Zhu
mouse	WB	Redox Biol; Regulation of respiratory complex I assembly by FMN cofactor targeting; Authors - Andrea Curtabbi
human	WB	Cancer Discov; Coordinated Transcriptional and Catabolic Programs Support Iron-Dependent Adaptation to RAS-MAPK Pathway Inhibition in Pancreatic Cancer; Authors - Mirunalini Ravichandran
human	WB	Front Oncol; Aurintricarboxylic acid inhibits the malignant phenotypes of drug-resistant cells via translation regulation; Authors - Keke Shang
human	WB	Cell Rep; Regional Molecular Mapping of Primate Synapses during Normal Healthy Aging.; Authors - Laura C Graham

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