Anticorps Polyclonal de lapin anti-NDUFS6

NDUFS6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14417-1-PBS

Synonymes

CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

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Galerie de données de validation

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  WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  WB analysis using 14417-1-AP (same clone as 14417-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 14417-1-AP (same clone as 14417-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 14417-1-AP (same clone as 14417-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14417-1-AP (same clone as 14417-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 14417-1-AP (same clone as 14417-1-PBS)

  IP result of anti-NDUFS6 (IP:14417-1-AP, 4ug; Detection:14417-1-AP 1:300) with mouse liver tissue lysate 3200 ug. This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14417-1-AP (same clone as 14417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14417-1-AP (same clone as 14417-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14417-1-AP (same clone as 14417-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS6 antibody (14417-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14417-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14417-1-PBS cible NDUFS6 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFS6 Protéine recombinante Ag5762
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC046155
• Symbole du gène: NDUFS6
• Identification du gène (NCBI): 4726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as 1CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

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