Anticorps Monoclonal anti-NDUFS6

NDUFS6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, poulet, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F4H3

N° de cat : 68329-1-PBS

Synonymes

2F4H3, CI 13kD A, CI-13kD-A, Complex I 13kD A, Complex I-13kD-A

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Galerie de données de validation

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  WB analysis of rabbit heart using 68329-1-Ig (same clone as 68329-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68329-1-Ig (NDUFS6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68329-1-Ig (NDUFS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68329-1-Ig (same clone as 68329-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS6 antibody (68329-1-Ig, Clone: 2F4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68329-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68329-1-PBS cible NDUFS6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, poulet, rat, souris

• Réactivité: Humain, Lapin, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFS6 Protéine recombinante Ag6286
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: BC046155
• Symbole du gène: NDUFS6
• Identification du gène (NCBI): 4726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

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