Anticorps Monoclonal anti-NDUFV1

NDUFV1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E9D4

N° de cat : 68144-1-PBS

Synonymes

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

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Galerie de données de validation

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  WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68144-1-Ig (same clone as 68144-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 68144-1-Ig (same clone as 68144-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68144-1-Ig (NDUFV1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68144-1-Ig (same clone as 68144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68144-1-Ig (same clone as 68144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68144-1-Ig (NDUFV1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68144-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68144-1-PBS cible NDUFV1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFV1 Protéine recombinante Ag32659
• Nom complet: NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC015645
• Symbole du gène: NDUFV1
• Identification du gène (NCBI): 4723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

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