Anticorps Polyclonal de lapin anti-NDUFV2

• Phare
• Validé par KD/KO

NDUFV2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

38

N° de cat : 15301-1-AP

Synonymes

NDUFV2

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Galerie de données de validation

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  WB analysis using 15301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15301-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 15301-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15301-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15301-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15301-1-AP

  IP Result of anti-NDUFV2 (IP:15301-1-AP, 3ug; Detection:15301-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15301-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15301-1-AP (NDUFV2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with alpha tubulin antibody 66031-1-Ig and CoraLite®594-Conjugated AffiniPure Goat Anti-mouse IgG(H+L). Blue: DAPI.

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  IF Staining of HeLa using 15301-1-AP

  Immunofluorescent analysis of Hela cells, using NDUFV2 antibody 15301-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Informations sur le produit

15301-1-AP cible NDUFV2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFV2 Protéine recombinante Ag7559
• Nom complet: NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 24-27 kDa
• Numéro d’acquisition GenBank: BC001632
• Symbole du gène: NDUFV2
• Identification du gène (NCBI): 4729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human,mouse	WB,IP	Nat Commun; A salvage pathway maintains highly functional respiratory complex I.; Authors - Karolina Szczepanowska
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
mouse	WB	Nat Commun; Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice.; Authors - Satomi Miwa
mouse	WB	J Cell Biol; A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity.; Authors - Vinod Kumar