Anticorps Monoclonal anti-NDUFV2

NDUFV2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B4A5

N° de cat : 68150-1-PBS

Synonymes

NDUFV2

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Galerie de données de validation

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  WB analysis using 68150-1-Ig (same clone as 68150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of chicken heart using 68150-1-Ig (same clone as 68150-1-PBS)

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68150-1-Ig (same clone as 68150-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68150-1-Ig (NDUFV2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 68150-1-Ig (same clone as 68150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68150-1-Ig (NDUFV2 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68150-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68150-1-PBS cible NDUFV2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFV2 Protéine recombinante Ag7760
• Nom complet: NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 24-27 kDa
• Numéro d’acquisition GenBank: BC001632
• Symbole du gène: NDUFV2
• Identification du gène (NCBI): 4729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH: ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

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