Anticorps Polyclonal de lapin anti-NEBL

NEBL Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21497-1-PBS

Synonymes

Nebulette, LNEBL, C10orf113, Actin-binding Z-disk protein, Actin binding Z disk protein

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Galerie de données de validation

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  WB analysis using 21497-1-AP (same clone as 21497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21497-1-AP (NEBL antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21497-1-AP (NEBL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 21497-1-AP (same clone as 21497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using NEBL antibody (21497-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21497-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21497-1-PBS cible NEBL dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEBL Protéine recombinante Ag15882
• Nom complet: nebulette
• Masse moléculaire calculée: 1014 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC110453
• Symbole du gène: NEBL
• Identification du gène (NCBI): 10529
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nebulette, also known as NEBL, is a cardiac-specific isoform belonging to the nebulin family of proteins. Nebulette has 23 modular repeat structures of 35 amino acid residues each. Nebulette has an acidic region with unknown structure at its N-terminus, and a serine-rich region adjacent to an SH3 domain at its C-terminus (PMID: 2037050, 20951588). Nebulette functionally links sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Nebulette is abundantly expressed in cardiac muscle at Z-disc regions, but not in skeletal or smooth muscle.

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