Anticorps Polyclonal de lapin anti-NEDD1

NEDD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13993-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 13993-1-AP (same clone as 13993-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13993-1-AP (same clone as 13993-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 13993-1-AP (same clone as 13993-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13993-1-AP (same clone as 13993-1-PBS)

  IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug. This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13993-1-AP (same clone as 13993-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13993-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13993-1-PBS cible NEDD1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEDD1 Protéine recombinante Ag5077
• Nom complet: neural precursor cell expressed, developmentally down-regulated 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 75 kDa, 62 kDa
• Numéro d’acquisition GenBank: BC027605
• Symbole du gène: NEDD1
• Identification du gène (NCBI): 121441
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.

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