Anticorps Recombinant de lapin anti-NEDD4

NEDD4 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230478F1

N° de cat : 83112-1-RR

Synonymes

KIAA0093, HECT-type E3 ubiquitin transferase NEDD4, EC:2.3.2.26, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein

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Galerie de données de validation

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  WB analysis using 83112-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83112-1-RR (NEDD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 83112-1-RR

  IP result of anti-NEDD4 (IP:83112-1-RR, 4ug; Detection:83112-1-RR 1:1000) with NIH/3T3 cells lysate 1505 ug.

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  IF Staining of U2OS using 83112-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using NEDD4 antibody (83112-1-RR, Clone: 230478F1 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83112-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83112-1-RR against Human NEDD4 were performed. The affinity constant is 14.8 nM.

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Applications testées

• Résultats positifs en WB: cellules C6, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83112-1-RR cible NEDD4 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NEDD4 Protéine recombinante Ag16291
• Nom complet: neural precursor cell expressed, developmentally down-regulated 4
• Masse moléculaire calculée: 1319 aa, 149 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC152452
• Symbole du gène: NEDD4
• Identification du gène (NCBI): 4734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

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