Anticorps Recombinant de lapin anti-NEDD4

NEDD4 Recombinant Antibody for WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230478F1

N° de cat : CL750-83112

Synonymes

230478F1, Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4

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Galerie de données de validation

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  WB analysis using CL750-83112

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83112 (NEDD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-83112 cible NEDD4 dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NEDD4 Protéine recombinante Ag16291
• Nom complet: neural precursor cell expressed, developmentally down-regulated 4
• Masse moléculaire calculée: 1319 aa, 149 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC152452
• Symbole du gène: NEDD4
• Identification du gène (NCBI): 4734
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

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