Anticorps Recombinant de lapin anti-NEDD9

• Phare
• Validé par KD/KO

NEDD9 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240310F8

N° de cat : 83362-2-RR

Synonymes

HEF1, Enhancer of filamentation 1 p55, CRK-associated substrate-related protein, CAS-L, CASL

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Galerie de données de validation

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  WB analysis using 83362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83362-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83362-2-RR (NEDD9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83362-2-RR

  WB result of NEDD9 antibody (83362-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD9 transfected HepG2 cells.

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  IF Staining of MCF-7 using 83362-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 83362-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NEDD9 antibody (83362-2-RR, Clone: 240310F8 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 83362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83362-2 against Human NEDD9 were performed. The affinity constant is 0.173 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules Raji, cellules U-251
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83362-2-RR cible NEDD9 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NEDD9 Protéine recombinante Ag34170
• Nom complet: neural precursor cell expressed, developmentally down-regulated 9
• Masse moléculaire calculée: 834 aa, 93 kDa
• Poids moléculaire observé: 105 kDa, 115 kDa
• Numéro d’acquisition GenBank: BC040207
• Symbole du gène: NEDD9
• Identification du gène (NCBI): 4739
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD9, also known as CASL, HEF1, contains an N-terminal Src homology (SH) 3 domain and an adjacent domain composed of multiple SH2-binding motifs(PMID: 8668148). Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. p105 and p115 are predominantly cytoplasmic and associated with focal adhesions, whereas p55associates with the mitotic spindle(PMID: 9584194).

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