Anticorps Polyclonal de lapin anti-NF-H/NF200

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(85)

N° de cat : 18934-1-AP

Synonymes

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 200 kDa neurofilament protein



Applications testées

Résultats positifs en WBtissu cérébral de souris, tissu cérébral de rat
Résultats positifs en IHCtissu cérébral de rat, tissu de gliome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu de cervelet de rat, tissu cérébral de rat, tissu cérébral de souris
Résultats positifs en IF-Frotissu cérébral de souris,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochimie (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

18934-1-AP cible NF-H/NF200 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, canin, Humain, singe, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NF-H/NF200 Protéine recombinante Ag13517
Nom complet neurofilament, heavy polypeptide
Masse moléculaire calculée 112 kDa
Poids moléculaire observé 200 kDa, 140-160 kDa
Numéro d’acquisition GenBankBC014185
Symbole du gène NF-H
Identification du gène (NCBI) 4744
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

Protocole

Product Specific Protocols
WB protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IHC protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IF protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
mouseIF

Adv Sci (Weinh)

GPR37 Activation Alleviates Bone Cancer Pain via the Inhibition of Osteoclastogenesis and Neuronal Hyperexcitability

Authors - Kaiyuan Wang
humanIHC

Small Methods

Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

Authors - Shunyi Lu
ratIHC

Biofabrication

Pulsed electromagnetic field-assisted reduced graphene oxide composite 3D printed nerve scaffold promotes sciatic nerve regeneration in rats

Authors - Zichao Wang
ratIF

Cell Rep

TMC7 functions as a suppressor of Piezo2 in primary sensory neurons blunting peripheral mechanotransduction

Authors - Xiaoxue Zhang

Acta Neuropathol Commun

A robust TDP-43 knock-in mouse model of ALS.

Authors - Shih-Ling Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

aidan (Verified Customer) (07-27-2024)

icc on retinal neurons (tried both mouse and human - human in pic). worked very well. would highly recommend

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: retinal neuron (mouse and human)
NF-H/NF200 Antibody Immunofluorescence validation (1:500 dilution) in retinal neuron (mouse and human) (Cat no:18934-1-AP)
FH

Paula (Verified Customer) (04-17-2024)

Works well for immunocytochemistry. Cells after neurogenic differentiation - image attached.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
NF-H/NF200 Antibody Immunofluorescence validation (1:500 dilution) in (Cat no:18934-1-AP)
FH

Azita (Verified Customer) (06-02-2021)

Western blotting analysis of NSC34 cells using NF-H Polyclonal Antibody showed strong bonds when the antibody was used at the dilution of 1:400.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: NSC34 cells
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