Anticorps Polyclonal de lapin anti-NF-M

NF-M Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 25805-1-AP

Synonymes

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

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Galerie de données de validation

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  WB analysis using 25805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25805-1-AP (NF-M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25805-1-AP (NEFM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25805-1-AP (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 25805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25805-1-AP (NEFM Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 25805-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25805-1-AP (NF-M antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-12 using 25805-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (25805-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu cérébral humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

25805-1-AP cible NF-M dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-M Protéine recombinante Ag22709
• Nom complet: neurofilament, medium polypeptide
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC002421
• Symbole du gène: NEFM
• Identification du gène (NCBI): 4741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognizes endogenous NF-M protein.

Publications

Species	Application	Title
canine	IF	Theranostics; Implantation of regenerative complexes in traumatic brain injury canine models enhances the reconstruction of neural networks and motor function recovery.; Authors - Jipeng Jiang
mouse, canine	IF	Biomater Sci; 3D printing collagen/heparin sulfate scaffolds boost neural network reconstruction and motor function recovery after traumatic brain injury in canine.; Authors - Jipeng Jiang
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
canine	IHC	Front Cardiovasc Med; Vagus Nerve Stimulation in Early Stage of Acute Myocardial Infarction Prevent Ventricular Arrhythmias and Cardiac Remodeling.; Authors - Shuang Zhao
rat	WB	Neurosci Lett; Overexpression of miR-338-5p in exosomes derived from mesenchymal stromal cells provides neuroprotective effects by the Cnr1/Rap1/Akt pathway after spinal cord injury in rats.; Authors - Anwei Zhang
rat	WB	Cell Biol Int; Study of the protective effect on damaged intestinal epithelial cells of rat multilineage-differentiating stress-enduring (Muse) cells.; Authors - Dong Sun

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