Anticorps Monoclonal anti-NF-M

NF-M Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2E3B12

N° de cat : 66396-1-Ig

Synonymes

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

---

Galerie de données de validation

• 

  WB analysis of rat brain using 66396-1-Ig

  Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66396-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of PC-12 using 66396-1-Ig

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules PC-12, cerveau de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:200-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66396-1-Ig cible NF-M dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-M Protéine recombinante Ag22709
• Nom complet: neurofilament, medium polypeptide
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC002421
• Symbole du gène: NEFM
• Identification du gène (NCBI): 4741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.

Produits associés

NF-M Polyclonal antibody

25805-1-AP

NF-M-Specific Polyclonal antibody

20664-1-AP

NF-H/NF200 Polyclonal antibody

21471-1-AP

NF-H/NF200 Monoclonal antibody

60331-1-Ig

NF-L Polyclonal antibody

12998-1-AP

NF-L Monoclonal antibody

60189-1-Ig