Anticorps Polyclonal de lapin anti-NF-M-Specific

NF-M-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20664-1-AP

Synonymes

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M-Specific

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Galerie de données de validation

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  WB analysis of mouse brain using 20664-1-AP

  mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 20664-1-AP

  WB result of 20664-1-AP.

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  WB analysis of rat brain using 20664-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20664-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 20664-1-AP

  IP Result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of rat brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

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  IHC staining of rat brain using 20664-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.

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  IF Staining of SH-SY5Y using 20664-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-12 using 20664-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20664-1-AP cible NF-M-Specific dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: neurofilament, medium polypeptide
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: NM_005382
• Symbole du gène: NEFM
• Identification du gène (NCBI): 4741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. The antibody is specific to NEFM.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Neurofilament Light Regulates Axon Caliber, Synaptic Activity, and Organelle Trafficking in Cultured Human Motor Neurons.; Authors - Markus T Sainio
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu

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