Anticorps Polyclonal de lapin anti-NEIL3

• Phare
• Validé par KD/KO

NEIL3 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11621-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11621-1-AP (same clone as 11621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skin using 11621-1-AP (same clone as 11621-1-PBS)

  human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11621-1-AP (same clone as 11621-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 11621-1-AP (same clone as 11621-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skin using 11621-1-AP (same clone as 11621-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11621-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11621-1-PBS cible NEIL3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEIL3 Protéine recombinante Ag2200
• Nom complet: nei endonuclease VIII-like 3 (E. coli)
• Masse moléculaire calculée: 605 aa, 68 kDa
• Poids moléculaire observé: 62-68 kDa
• Numéro d’acquisition GenBank: BC025954
• Symbole du gène: NEIL3
• Identification du gène (NCBI): 55247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

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