Anticorps Monoclonal anti-NEK2

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D7B4

N° de cat : 66632-1-PBS

Synonymes

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

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Galerie de données de validation

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  WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of rat thymus using 66632-1-Ig (same clone as 66632-1-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66632-1-Ig (same clone as 66632-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66632-1-Ig (same clone as 66632-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66632-1-Ig (same clone as 66632-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66632-1-Ig (same clone as 66632-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66632-1-Ig (same clone as 66632-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66632-1-PBS cible NEK2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEK2 Protéine recombinante Ag21476
• Nom complet: NIMA (never in mitosis gene a)-related kinase 2
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC043502
• Symbole du gène: NEK2
• Identification du gène (NCBI): 4751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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