Anticorps Polyclonal de lapin anti-NEK4

NEK4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31389-1-PBS

Synonymes

STK2, pp12301, NRK2, NimA related protein kinase 4

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Galerie de données de validation

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  WB analysis using 31389-1-AP (same clone as 31389-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31389-1-AP (NEK4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31389-1-AP (same clone as 31389-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31389-1-AP (NEK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31389-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31389-1-PBS cible NEK4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEK4 Protéine recombinante Ag34929
• Nom complet: NIMA (never in mitosis gene a)-related kinase 4
• Masse moléculaire calculée: 781 aa, 88 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC063044
• Symbole du gène: NEK4
• Identification du gène (NCBI): 6787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.

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