Anticorps Polyclonal de lapin anti-NEK7

NEK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 31422-1-AP

Synonymes

NimA related protein kinase 7

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Galerie de données de validation

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  WB analysis using 31422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31422-1-AP (NEK7 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 31422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 31422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31422-1-AP (NEK7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEK7 antibody (31422-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HeLa, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu cardiaque de souris, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31422-1-AP cible NEK7 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEK7 Protéine recombinante Ag33629
• Nom complet: NIMA (never in mitosis gene a)-related kinase 7
• Masse moléculaire calculée: 35
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: NM_133494
• Symbole du gène: NEK7
• Identification du gène (NCBI): 140609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian NIMA-related kinases (NEKs) represent a family of serine/threonine kinases, named NEK1-NEK11, which are implicated in the control of several aspects of mitosis and are involved in non-mitotic functions (PMID: 33364979). NIMA-related kinase 7 (NEK7) is the smallest NIMA-related kinase (NEK) in mammals. NEK7 is identified as a highly conserved serine or threonine kinase, which is not only crucial for mitosis entry, cell cycle progression, cell division, and mitotic process, but also expressed in brain, heart, lung, liver, spleen, and other issues (PMID: 31787755).

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