Anticorps Polyclonal de lapin anti-NELF-A

• Phare
• Validé par KD/KO

NELF-A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10456-1-PBS

Synonymes

WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A

---

Galerie de données de validation

• 

  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10456-1-AP (same clone as 10456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 10456-1-AP (same clone as 10456-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10456-1-AP (same clone as 10456-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)

  IP result of anti-NELF-A (IP:10456-1-AP, 4ug; Detection:10456-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10456-1-AP (same clone as 10456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELF-A antibody (10456-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10456-1-PBS cible NELF-A dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NELF-A Protéine recombinante Ag0708
• Nom complet: Wolf-Hirschhorn syndrome candidate 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC002764
• Symbole du gène: NELFA
• Identification du gène (NCBI): 7469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].

{{ptg:RelatedPrimaryAntibodies}}