Anticorps Recombinant de lapin anti-NELFB

NELFB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241434F12

N° de cat : 84135-4-RR

Synonymes

NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1, COBRA1

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Galerie de données de validation

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  WB analysis using 84135-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84135-4-RR (NELF-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84135-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NELF-B antibody (84135-4-RR, Clone: 241434F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 84135-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of BxPC-3 using 84135-4-RR

  1x10^6 BxPC-3 cells were intracellularly stained with 0.25 ug NELF-B Recombinant antibody (84135-4-RR, Clone:241434F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84135-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84135-4-RR against Human NELF-B were performed. The affinity constant is 0.199 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules BxPC-3, cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431, cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84135-4-RR cible NELFB dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NELFB Protéine recombinante Ag9599
• Nom complet: cofactor of BRCA1
• Masse moléculaire calculée: 580aa,66 kDa; 475aa,54 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC011892
• Symbole du gène: NELFB
• Identification du gène (NCBI): 25920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

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