Anticorps Polyclonal de lapin anti-NESG1

NESG1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21077-1-AP

Synonymes

CCDC19, CFAP45

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Galerie de données de validation

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  WB analysis of rat testis using 21077-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21077-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

21077-1-AP cible NESG1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NESG1 Protéine recombinante Ag15257
• Nom complet: coiled-coil domain containing 19
• Masse moléculaire calculée: 551 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC027874
• Symbole du gène: CCDC19
• Identification du gène (NCBI): 25790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC19, also named as NESG1, is specifically expressed in human nasopharynx and trachea, and decreased or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines. In addition, the levels of NESG1 protein are significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. NESG1 also significantly regulats the expression of cell cycle regulators CCNA1 and p21. NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma.; Authors - X Yu
human	WB,IHC	J Cell Mol Med; Candidate tumour suppressor CCDC19 regulates miR-184 direct targeting of C-Myc thereby suppressing cell growth in non-small cell lung cancers.; Authors - Zhen Liu ; KD Validated

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