Anticorps Polyclonal de lapin anti-NET1

• Phare
• Validé par KD/KO

NET1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 28180-1-AP

Synonymes

Proto-oncogene p65 Net1, Proto oncogene p65 Net1, Neuroepithelial cell-transforming gene 1 protein, NET1A, ARHGEF8

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Galerie de données de validation

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  WB analysis using 28180-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 28180-1-AP (NET1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 28180-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28180-1-AP (NET1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 28180-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28180-1-AP (NET1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28180-1-AP (NET1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28180-1-AP (NET1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 28180-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NET1 antibody (28180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

28180-1-AP cible NET1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NET1 Protéine recombinante Ag28142
• Nom complet: neuroepithelial cell transforming 1
• Masse moléculaire calculée: 596 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC010285
• Symbole du gène: NET1
• Identification du gène (NCBI): 10276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Gastric Cancer; LncRNA CTC-497E21.4 promotes the progression of gastric cancer via modulating miR-22/NET1 axis through RhoA signaling pathway.; Authors - Wei Zong
mouse	WB,IF	Reprod Biol Endocrinol; NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes; Authors - Shiwei Wang ; KD Validated
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
human,mouse	WB,IHC,IF	Gastroenterology; Cell-in-Cell-Mediated Entosis Reveals A Progressive Mechanism in Pancreatic Cancer; Authors - Jianlu Song ; KD Validated
mouse	WB,IF	iScience; Neuroepithelial cell-transforming 1 promotes cardiac fibrosis via the Wnt/β-catenin signaling pathway; Authors - Tianyu Li ; KD Validated
mouse	WB,IF	Theriogenology; Reduced NET1 adversely affects early embryonic development in mice; Authors - Shiwei Wang ; KD Validated

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