Anticorps Recombinant de lapin anti-NETO2

• Phare
• Validé par KD/KO

NETO2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241451E10

N° de cat : 84222-2-RR

Synonymes

241451E10, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, BTCL2, NEOT2, Neuropilin and tolloid-like protein 2

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Galerie de données de validation

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  WB analysis using 84222-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84222-2-RR (NETO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84222-2-RR

  WB result of NETO2 antibody (84222-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NETO2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 84222-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84222-2-RR against Human NETO2 were performed. The affinity constant is 0.203 nM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HEK-293, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

84222-2-RR cible NETO2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NETO2 Protéine recombinante Ag2971
• Nom complet: neuropilin (NRP) and tolloid (TLL)-like 2
• Masse moléculaire calculée: 525 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC012381
• Symbole du gène: NETO2
• Identification du gène (NCBI): 81831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuropilin and tolloid-like 2 (NETO2), a member of the subfamily of CUB domain and LDLa-containing proteins, was identified as an auxiliary protein of neuronal kainate receptors (KARs) and played critical roles in regulating the functions of KARs. It was also able to bind to the active oligomeric form of K+-Cl− cotransporter (KCC2) to enhance its recycling in hippocampal neurons. Recently, elevated mRNA levels of NETO2 were detected in several types of tumors.

Publications

Species	Application	Title
human	WB	World J Surg Oncol; Targeting NETO2 suppresses cell proliferation, invasion, and migration and inactivates the STAT3/C-MYC pathway in hepatocellular carcinoma; Authors - Na Shun Meng He

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