Anticorps Monoclonal anti-NEU1

NEU1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F6D1

N° de cat : 67032-1-PBS

Synonymes

1F6D1, Acetylneuraminyl hydrolase, EC:3.2.1.18, G9 sialidase, Lysosomal sialidase

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Galerie de données de validation

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  WB analysis of PC-12 using 67032-1-Ig (same clone as 67032-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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  WB analysis using 67032-1-Ig (same clone as 67032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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  WB analysis of rat pancreas using 67032-1-Ig (same clone as 67032-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67032-1-Ig (same clone as 67032-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67032-1-Ig (same clone as 67032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67032-1-Ig (NEU1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 67032-1-Ig (same clone as 67032-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 67032-1-Ig (NEU1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67032-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67032-1-PBS cible NEU1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEU1 Protéine recombinante Ag27945
• Nom complet: sialidase 1 (lysosomal sialidase)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC011900
• Symbole du gène: NEU1
• Identification du gène (NCBI): 4758
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

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