Anticorps Recombinant de lapin anti-NEU2

NEU2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240685B3

N° de cat : 83934-1-PBS

Synonymes

240685B3, Cytosolic sialidase, EC:3.2.1.18, N acetyl alpha neuraminidase 2, N-acetyl-alpha-neuraminidase 2

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 83934-1-RR (NEU2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 83934-1-RR (same clone as 83934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83934-1-RR (NEU2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83934-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83934-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83934-1-RR against Human NEU2 were performed. The affinity constant is 2.76 nM.

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Informations sur le produit

83934-1-PBS cible NEU2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NEU2 Protéine recombinante Ag17386
• Nom complet: sialidase 2 (cytosolic sialidase)
• Masse moléculaire calculée: 380 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC107053
• Symbole du gène: NEU2
• Identification du gène (NCBI): 4759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEU2, also named as Sialidase 2, neuraminidase 2, catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosaccharides. NEU2 is localized in the cytosol and plasma membrane and takes part in myoblast and neuronal differentiation. It is almost undetectable under normal physiological conditions, and was found to be involved into apoptotic events in pancreatic ductal adenocarcinoma, one of the most dangerous cancers due to poor diagnosis and prognosis. (PMID: 30439363)

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