Anticorps Polyclonal de lapin anti-NEUROD1

• Phare
• Validé par KD/KO

NEUROD1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

Avis (2)

N° de cat : 12081-1-AP

Synonymes

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3

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Galerie de données de validation

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  WB analysis using 12081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12081-1-AP (NEUROD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12081-1-AP

  IP result of anti-NEUROD1 (IP:12081-1-AP, 3ug; Detection:12081-1-AP 1:200) with Y79 cells lysate 2000ug.

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  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IP: cellules Y79
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

12081-1-AP cible NEUROD1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEUROD1 Protéine recombinante Ag2713
• Nom complet: neurogenic differentiation 1
• Masse moléculaire calculée: 356 aa, 40 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC009046
• Symbole du gène: NEUROD1
• Identification du gène (NCBI): 4760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer.; Authors - Ayushi S Patel
mouse	IF	Sci Adv; Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration; Authors - Gwyneth M Welch
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	WB	Proc Natl Acad Sci U S A; CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation.; Authors - Sabina Sood
mouse	IHC	Cell Rep; PMID: 31189116; Authors - Franziska Böttger
mouse	IF	PLoS Biol; Centrioles are amplified in cycling progenitors of olfactory sensory neurons.; Authors - Kaitlin Ching

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (07-15-2025)

Immunohistofluorescence (PFA-fixed cryosections) image of NeuroD1 stained mouse brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Mouse Brain

NEUROD1 Antibody Immunofluorescence validation (1/200 dilution) in Mouse Brain (Cat no:12081-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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