Anticorps Monoclonal anti-NEUROD1

NEUROD1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3E10F1

N° de cat : 66691-1-PBS

Synonymes

3E10F1, bHLHa3, Class A basic helix-loop-helix protein 3, NEUROD

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Galerie de données de validation

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  WB analysis of Y79 using 66691-1-Ig (same clone as 66691-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66691-1-Ig (NEUROD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66691-1-Ig (same clone as 66691-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66691-1-Ig (NEUROD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66691-1-Ig (same clone as 66691-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.5 ug Anti-Human NEUROD1 (66691-1-Ig, Clone:3E10F1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66691-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66691-1-PBS cible NEUROD1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEUROD1 Protéine recombinante Ag27606
• Nom complet: neurogenic differentiation 1
• Masse moléculaire calculée: 356 aa, 40 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC009046
• Symbole du gène: NEUROD1
• Identification du gène (NCBI): 4760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

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