Anticorps Monoclonal anti-NEUROG1

NEUROG1 Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1E1H6

N° de cat : 66642-1-Ig

Synonymes

1E1H6, AKA, bHLHa6, Class A basic helix-loop-helix protein 6, Math4C

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Galerie de données de validation

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  WB analysis of HEK-293 using 66642-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 66642-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66642-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66642-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66642-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 66642-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NEUROG1 antibody (66642-1-Ig, Clone: 1E1H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Neuro-2a, cellules Y79, tissu cérébral de souris
• Résultats positifs en IF-P: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66642-1-Ig cible NEUROG1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEUROG1 Protéine recombinante Ag26438
• Nom complet: neurogenin 1
• Masse moléculaire calculée: 237 aa, 26 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008687
• Symbole du gène: NEUROG1
• Identification du gène (NCBI): 4762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

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