Anticorps Monoclonal anti-NF-κB p65

• Phare
• Validé par KD/KO

NF-κB p65 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B12D11

N° de cat : 66535-1-PBS

Synonymes

NF-kB, NFKB3, NF-κB, NF-κB p65, NF-κB3, p65, RELA, Transcription factor p65

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Galerie de données de validation

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  WB analysis of HEK-293 using 66535-1-Ig (same clone as 66535-1-PBS)

  WB result of p65; RELA antibody (66535-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p65; RELA transfected HEK-293 cells. This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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  WB analysis using 66535-1-Ig (same clone as 66535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66535-1-Ig (NF-κB p65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66535-1-Ig (same clone as 66535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66535-1-Ig (same clone as 66535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66535-1-Ig (same clone as 66535-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF-κB p65 antibody (66535-1-Ig, Clone: 1B12D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66535-1-PBS cible NF-κB p65 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-κB p65 Protéine recombinante Ag1199
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: NF-κB p65
• Identification du gène (NCBI): 5970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

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