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Anticorps Recombinant de lapin anti-NF-κB p65

NF-κB p65 Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(112)

CloneNo.

4C7

N° de cat : 80979-1-RR

Synonymes

NF κB, p65, p65; RELA, RELA, 4C7



Applications testées

Résultats positifs en WBcellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules MOLT-4, cellules NIH/3T3, cellules Raji
Résultats positifs en IPcellules HeLa,
Résultats positifs en IF/ICCcellules HepG2, TNF alpha treated HT-1080 cells
Résultats positifs en FC (Intra)cellules HepG2
Résultats positifs en ChIP-qPCRhTNF-α-HeLa, 30 ng/ml, 1 h HeLa cells,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
CHImmunoprécipitation (IP)-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

80979-1-RR cible NF-κB p65 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène NF-κB p65 Protéine recombinante Ag1199
Nom complet v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Masse moléculaire calculée 65 kDa
Poids moléculaire observé65 kDa
Numéro d’acquisition GenBankBC011603
Symbole du gène NF-κB p65
Identification du gène (NCBI) 5970
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit monoclonal antibody raised against residues near the N terminus of human RELA.

Protocole

Product Specific Protocols
WB protocol for NF-κB p65 antibody 80979-1-RRDownload protocol
IF protocol for NF-κB p65 antibody 80979-1-RRDownload protocol
IP protocol for NF-κB p65 antibody 80979-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Adv Sci (Weinh)

Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution

Authors - Rui Wang
mouseWB

Small

Polymer-Encapsulated Catalase for Targeted Redox Regulation in Acute Liver Injury

Authors - Feifei Li
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
mouse

J Exp Clin Cancer Res

METTL3 recruiting M2-type immunosuppressed macrophages by targeting m6A-SNAIL-CXCL2 axis to promote colorectal cancer pulmonary metastasis

Authors - Peng Ouyang
mouseWB

Carbohydr Polym

Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata

Authors - Wei Xu
mouseWB

Sci Total Environ

Effects of chronic low-level lead (Pb) exposure on cognitive function and hippocampal neuronal ferroptosis: An integrative approach using bioinformatics analysis, machine learning, and experimental validation

Authors - Yingsi Cao
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