- Phare
- Validé par KD/KO
Anticorps Recombinant de lapin anti-NF-κB p65
NF-κB p65 Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR
Conjugaison
Non conjugué
CloneNo.
4C7
N° de cat : 80979-1-RR
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules MOLT-4, cellules NIH/3T3, cellules Raji |
Résultats positifs en IP | cellules HeLa, |
Résultats positifs en IF/ICC | cellules HepG2, TNF alpha treated HT-1080 cells |
Résultats positifs en FC (Intra) | cellules HepG2 |
Résultats positifs en ChIP-qPCR | hTNF-α-HeLa, 30 ng/ml, 1 h HeLa cells, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:40000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
CHImmunoprécipitation (IP)-QPCR | CHIP-QPCR : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 100 publications below |
IHC | See 1 publications below |
IF | See 22 publications below |
Informations sur le produit
80979-1-RR cible NF-κB p65 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, porc, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Recombinant |
Type | Anticorps |
Immunogène | NF-κB p65 Protéine recombinante Ag1199 |
Nom complet | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
Masse moléculaire calculée | 65 kDa |
Poids moléculaire observé | 65 kDa |
Numéro d’acquisition GenBank | BC011603 |
Symbole du gène | NF-κB p65 |
Identification du gène (NCBI) | 5970 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit monoclonal antibody raised against residues near the N terminus of human RELA.
Protocole
Product Specific Protocols | |
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WB protocol for NF-κB p65 antibody 80979-1-RR | Download protocol |
IF protocol for NF-κB p65 antibody 80979-1-RR | Download protocol |
IP protocol for NF-κB p65 antibody 80979-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution | ||
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
J Exp Clin Cancer Res METTL3 recruiting M2-type immunosuppressed macrophages by targeting m6A-SNAIL-CXCL2 axis to promote colorectal cancer pulmonary metastasis | ||
Carbohydr Polym Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata | ||
Sci Total Environ Effects of chronic low-level lead (Pb) exposure on cognitive function and hippocampal neuronal ferroptosis: An integrative approach using bioinformatics analysis, machine learning, and experimental validation |