Anticorps Polyclonal de lapin anti-NF-κB p65

• Phare
• Validé par KD/KO

NF-κB p65 Polyclonal Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(2)

N° de cat : CL594-10745

Synonymes

NF κB, p65, p65; RELA, RELA, NF Kappa B

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green). DAPI (Blue).

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  IF Staining of HT-1080 using CL594-10745

  Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2, TNF alpha treated HT-1080 cells

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL594-10745 cible NF-κB p65 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-κB p65 Protéine recombinante Ag1199
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: NF-κB p65
• Identification du gène (NCBI): 5970
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Publications

Species	Application	Title
mouse	IF	Front Med (Lausanne); New, potent, small molecule agonists of tyrosine kinase receptors attenuate dry eye disease; Authors - Zhiyuan Yu
mouse	IF	Invest Ophthalmol Vis Sci; Induction of Innate Inflammatory Pathways in the Corneal Epithelium in the Desiccating Stress Dry Eye Model; Authors - Zhiyuan Yu

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