Anticorps Polyclonal de lapin anti-NF-H/NF200

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18934-1-PBS

Synonymes

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 200 kDa neurofilament protein

---

Galerie de données de validation

• 

  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

  WB result of 18934-1-AP. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 GFAP antibody (CL594-60190, Clone: 4B2E10, red), NeuN antibody (66836-1-Ig, Clone: 3A4C1, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18934-1-PBS cible NF-H/NF200 dans les applications de WB, IHC, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF-H/NF200 Protéine recombinante Ag13517
• Nom complet: neurofilament, heavy polypeptide
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 200 kDa, 140-160 kDa
• Numéro d’acquisition GenBank: BC014185
• Symbole du gène: NF-H
• Identification du gène (NCBI): 4744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

{{ptg:RelatedPrimaryAntibodies}}