Anticorps Monoclonal anti-NF-H/NF200

NF-H/NF200 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1A3C7

N° de cat : 60331-1-PBS

Synonymes

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 1A3C7

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Galerie de données de validation

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  WB analysis of pig cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 60331-1-Ig (same clone as 60331-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 60331-1-Ig (same clone as 60331-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-H/NF200 (60331-1-Ig, Clone:1A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60331-1-PBS cible NF-H/NF200 dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-H/NF200 Protéine recombinante Ag13517
• Nom complet: neurofilament, heavy polypeptide
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC014185
• Symbole du gène: NF-H
• Identification du gène (NCBI): 4744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.

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