Anticorps Monoclonal anti-NF-L

NF-L Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5C12G4

N° de cat : 60189-1-PBS

Synonymes

NEFL, 5C12G4, neurofilament, NF68, NFL

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Galerie de données de validation

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  WB analysis using 60189-1-Ig (same clone as 60189-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60189-1-Ig (same clone as 60189-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-L antibody (60189-1-Ig, Clone: 5C12G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 60189-1-Ig (same clone as 60189-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60189-1-PBS cible NF-L dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-L Protéine recombinante Ag15178
• Nom complet: neurofilament, light polypeptide
• Masse moléculaire calculée: 543 aa, 62 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC039237
• Symbole du gène: NF-L
• Identification du gène (NCBI): 4747
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

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