Anticorps Polyclonal de lapin anti-NF2

NF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 21686-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis using 21686-1-AP (same clone as 21686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 21686-1-AP (same clone as 21686-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 21686-1-AP (same clone as 21686-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21686-1-AP (same clone as 21686-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 21686-1-AP (same clone as 21686-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 21686-1-AP (same clone as 21686-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 21686-1-AP (same clone as 21686-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

  IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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  IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21686-1-PBS cible NF2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF2 Protéine recombinante Ag16265
• Nom complet: neurofibromin 2 (merlin)
• Masse moléculaire calculée: 595 aa, 70 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC020257
• Symbole du gène: NF2
• Identification du gène (NCBI): 4771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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