Anticorps Polyclonal de lapin anti-NF45

• Phare
• Validé par KD/KO

NF45 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 14714-1-AP

Synonymes

ILF2, Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa, PRO3063

---

Galerie de données de validation

• 

  WB analysis using 14714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14714-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14714-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14714-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14714-1-AP (NF45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14714-1-AP (NF45 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14714-1-AP cible NF45 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF45 Protéine recombinante Ag6436
• Nom complet: interleukin enhancer binding factor 2, 45kDa
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC000382
• Symbole du gène: ILF2
• Identification du gène (NCBI): 3608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

Publications

Species	Application	Title
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
mouse,human	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
		Analyst; An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.; Authors - Yu Yanyan Y
human	WB,IF	Virol J; Interleukin-2 enhancer binding factor 2 interacts with the nsp9 or nsp2 of porcine reproductive and respiratory syndrome virus and exerts negatively regulatory effect on the viral replication.; Authors - Xuexia Wen
human	WB	J Exp Clin Cancer Res; LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex; Authors - Dandan Li
human	WB	Nat Cell Biol; Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing; Authors - Guokai Yan

{{ptg:RelatedPrimaryAntibodies}}