Anticorps Monoclonal anti-NF45

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A3A2

N° de cat : 67685-1-PBS

Synonymes

ILF2, NF45, PRO3063

---

Galerie de données de validation

• 

  WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67685-1-Ig (NF45 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)

  IP result of anti-NF45 (IP:67685-1-Ig, 4ug; Detection:67685-1-Ig 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF45 antibody (67685-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67685-1-PBS cible NF45 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF45 Protéine recombinante Ag30388
• Nom complet: interleukin enhancer binding factor 2, 45kDa
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000382
• Symbole du gène: ILF2
• Identification du gène (NCBI): 3608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

{{ptg:RelatedPrimaryAntibodies}}