Anticorps Polyclonal de lapin anti-NFAT5

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21713-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 21713-1-AP (same clone as 21713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21713-1-AP (same clone as 21713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFAT5 antibody (21713-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21713-1-PBS cible NFAT5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFAT5 Protéine recombinante Ag16341
• Nom complet: nuclear factor of activated T-cells 5, tonicity-responsive
• Masse moléculaire calculée: 1531 aa, 166 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC146765
• Symbole du gène: NFAT5
• Identification du gène (NCBI): 10725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

{{ptg:RelatedPrimaryAntibodies}}