Anticorps Monoclonal anti-NFATC1

• Phare
• Validé par KD/KO

NFATC1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(63)

CloneNo.

1E1B10

N° de cat : 66963-1-Ig

Synonymes

1E1B10, NFAT2, NFATc, NF-ATc, NF-ATc1

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Galerie de données de validation

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  WB analysis using 66963-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66963-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 66963-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 66963-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66963-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66963-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules Daudi, cellules MOLT-4, cellules Raji, cellules Ramos, cellules Romas, cellules U2OS
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 58 publications below
• IHC: See 2 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

66963-1-Ig cible NFATC1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFATC1 Protéine recombinante Ag13014
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
• Masse moléculaire calculée: 716aa,78 kDa; 943aa,101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC104753
• Symbole du gène: NFATC1
• Identification du gène (NCBI): 4772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer; Authors - Cancan Zheng
human	WB	Mol Ther; C5a-C5aR1 axis controls mitochondrial fission to promote podocyte injury in lupus nephritis; Authors - Baokui Ye
human	WB,IF	Pharmacol Res; Targeting CD38/ ADP-ribosyl cyclase as a novel therapeutic strategy for identification of three potent agonists for leukopenia treatment; Authors - Yuanzhi Liu
rat	WB,IP,IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
human	WB	Oncogene; Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.; Authors - Mengyi Lao
human	WB,IF	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin

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