Anticorps Monoclonal anti-NFATC2

NFATC2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F3B2

N° de cat : 66917-1-PBS

Synonymes

NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp

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Galerie de données de validation

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  WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66917-1-Ig (NFATC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 (66917-1-Ig, Clone:1F3B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66917-1-PBS cible NFATC2 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NFATC2 Protéine recombinante Ag17990
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• Masse moléculaire calculée: 925 aa, 100 kDa
• Poids moléculaire observé: 135-140 kDa
• Numéro d’acquisition GenBank: BC136418
• Symbole du gène: NFATC2
• Identification du gène (NCBI): 4773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

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